Journal: PLoS ONE

Article Title: Stem Cells Derived from Neonatal Mouse Kidney Generate Functional Proximal Tubule-Like Cells and Integrate into Developing Nephrons In Vitro

doi: 10.1371/journal.pone.0062953

Figure Lengend Snippet: ( A, B ) Immunostaining for Wt1 (red) and Pax2 (green) (A), or Wt1 (green) and laminin-111 (red) (B) shows the presence of condensed MM (pink arrowheads) surrounding the UB (white arrowheads) after 3 days of culture, thus demonstrating that the induction of the mesenchyme had taken place. ( C ) Immunostaining for megalin (green) and Pax2 (blue) at day 3 of rudiment culture reveals the presence of proximal tubules (open arrowheads). ( D ) Immunostaining for synaptopodin (green) demonstrates the presence of nascent podocytes within developing glomerular structures (open arrowheads) at 5 days of culture. Scale bars are 75 µm (A), 150 µm (B, C) and 100 µm (D).

Article Snippet: The following primary antibodies were used: mouse anti-Wt1 (1∶500, Upstate), rabbit anti-Pax2 (1∶200, Covance), mouse anti-laminin-111 (1∶500, Sigma) mouse anti-megalin (1∶200, Acris Antibodies) mouse anti-synaptopodin (ready to use, Acris), mouse anti-calbindin-28 (1∶200, Abcam).

Techniques: Immunostaining

Journal: PLoS ONE

Article Title: Stem Cells Derived from Neonatal Mouse Kidney Generate Functional Proximal Tubule-Like Cells and Integrate into Developing Nephrons In Vitro

doi: 10.1371/journal.pone.0062953

Figure Lengend Snippet: ( A , B ) Chimeric organoids were stained for megalin (green). Arrowheads indicate QD-labeled (white), megalin + KSCs within a nascent proximal tubule. Note that megalin is present on the apical surface of the nascent proximal tubule (dotted lines in B), thus recapitulating its normal expression pattern observed in vivo . ( C , D ) Chimeric organoids were stained for synaptopodin (green) and laminin-111 (red). Arrowheads point to QD-labeled (white), synaptopodin + KSCs within a nascent glomerulus. Scale bars are 20 µm (A, B) and 8 µm (C, D).

Article Snippet: The following primary antibodies were used: mouse anti-Wt1 (1∶500, Upstate), rabbit anti-Pax2 (1∶200, Covance), mouse anti-laminin-111 (1∶500, Sigma) mouse anti-megalin (1∶200, Acris Antibodies) mouse anti-synaptopodin (ready to use, Acris), mouse anti-calbindin-28 (1∶200, Abcam).

Techniques: Staining, Labeling, Expressing, In Vivo

Journal: Physiological Reports

Article Title: Cytoresistance after acute kidney injury is limited to the recovery period of proximal tubule integrity and possibly involves Hippo‐YAP signaling

doi: 10.14814/phy2.13310

Figure Lengend Snippet: Primary antibodies, their dilutions and suppliers

Article Snippet: Goat polyclonal anti‐megalin (P‐20) , 1:200 , Santa Cruz Biotechnology.

Techniques: Immunocytochemistry, Immunohistochemistry